MOLECULAR PATHOLOGY AND ADVANCED DIAGNOSTICS LABORATORY

Taking the Dublin descriptors into account, the expected learning outcomes are therefore:

1. Knowledge and understanding: At the end of the course, students will have acquired theoretical and scientific knowledge of the main laboratory diagnostic methods, with particular reference to molecular investigation techniques. They will also have an in-depth understanding of the molecular bases of diseases, particularly genetic and oncological ones, and will understand their pathogenetic mechanisms.

2. Ability to apply knowledge and understanding: Students will be able to apply their acquired knowledge to the interpretation of the results of the main laboratory diagnostic methods, particularly molecular ones. They will be able to correlate molecular alterations with pathological findings, especially in the context of genetic and oncological diseases, understanding the rationale behind the various diagnostic strategies.

3. Independent judgment: Students will have developed critical skills in evaluating data from laboratory investigations, being able to recognize the reliability of results, methodological limitations, and potential sources of error. They will be able to critically interpret diagnostic information and compare it with data in the scientific literature.

4. Communication skills: Students will be able to clearly and appropriately communicate information, results, and interpretations related to diagnostic methods and the molecular basis of diseases, using appropriate scientific terminology that is understandable to both specialists and non-specialists.

5. Learning skills: Students will have developed independent learning skills that will allow them to continuously update their knowledge in the field of laboratory diagnostics and the molecular biology of diseases, including through critical consultation of the scientific literature, in a constantly evolving field.

Patogenesi-Basi genetiche e molecolari delle malattie. Piccin. Autori Ruggero Pardi - Pier Paolo Di Fiore

- Describe the operating principle of droplet digital PCR and how it differs from standard PCR methods.

- Types of microarray methods and description of the hybridization principle

- Technical and amplification and sequencing characteristics of next-generation sequencers

- What are the main molecular pathways altered in colon cancer?

- What is the effect of different mutations in the EGFR gene in lung cancer?